Evaluation of Protective Role of Prebiotic Galacto-Oligosaccharides Against Colon Cancer

Naureen Naeem; Shoaib Ahmad  Siddiqi; Mona  Hassan; Fatima Syed

doi:10.54692/lgujls.2018.020226

Naureen Naeem
Shoaib Ahmad Siddiqi Department of Biology, Lahore Garrison University, Lahore, Pakistan
Mona Hassan Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Fatima Syed University of Veterinary and Animal Sciences Lahore, Pakistan

DOI: https://doi.org/10.54692/lgujls.2018.020226

Keywords: Prebiotics, Galactooligosaccharides, Cancer, Colon carcinogenesis, Galactosidase

Abstract

In the present study role of treated galacto-oligosaccharides (GOS) synthesized from lactose (GOS) was assessed against development of colon cancer. Research was carried out in two phases, production of prebiotic galacto-oligosaccharide and assessment of their protective role against colon cancer. Greatest percentage of enzyme activity was observed at 6.5 pH and 37oC. Transgalactosylation process was accepted at 37°C by the using of enzymes with three different concentrations. Male Sprague Dawley rats going to treated galactooligosaccharides groups and inulin group had significantly lowest aberrant crypt foci (ACF) counts per colon in contrast to controlled animals (G1 and G5). The animals body mass belonging to galacto-oligosaccharides (Group G2, G3 and G4) increased suggestively higher as associated to control group (G1). Present results were found to be defensive in contradiction of colon cancer. Though more research is needed at DNA level to explore the mutations at gene levels.

Author Biography

Naureen Naeem

Department of Home-Economics; Lahore Garrison University, Lahore.56000, Pakistan