Phytochemical and in Vitro Biological Profiling of Portulaca grandiflora Whole Plant Extracts

  • Hina Salahuddin Department of Zoology, Faculty of Life Sciences, University of Okara, Okara 56130 Pakistan
  • Riffat Batool Institute of Biochemistry and Biotechnology, PMAS-UAAR Rawalpindi, Pakistan
  • Sabeen Sabri Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130
  • Qaiser Mansoor Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan
  • Muhammad Shoaib Akhtar Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara 56130
  • Tariq Mahmood Department of Plant Sciences, Faculty of Biological Sciences, Quaid e Azam, University, Islamabad, Pakistan
Keywords: Phenolic, Flavonoid, Antioxidant, DPPH, antimicrobial


ABSTRACT: This study aimed to determine the phytochemicals and to examine the in vitro antibacterial, antifungal, anticancer and antioxidant potential of P. grandiflora. Crude methanolic, ethanolic and n-Hexane extracts of whole plant were used for this study. Antimicrobial properties were estimated through disk diffusion assay, antioxidant activity was determined by using 2,2-diphenyl-1-picryl-hydrazyl scavenging (DPPH), total antioxidant capability and reducing power assays. It was found that methanolic extract of P. grandiflora showed the highest zone of inhibition against Pseudomonas aeruginosa and Candida albicans i.e. 36 ±1mm and 18 ±1 mm respectively with MIC of 25 µg/ml. The highest amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content were found in methanolic extract of P. grandiflora i.e. 77 ± 0.68 µg GAE/mg and 63 ± 1.08 µg QE/mg extract respectiely. The significant DPPH scavenging activity (P<0.05) was recorded for methanolic extract of P. grandiflora (87%). Considerably greater antioxidant capacity (10 ± 1.18 µg AAE/mg) and reducing power (4.06 ± 0.18 µg AAE/mg) was observed in methanolic extract. MTT cell viability assay methanolic extract of P. grandiflora showed the IC50 of 42.70 µg/ml at 72h. It was concluded this plant extracts exhibit strong pharmacological properties.

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